Analysis of post-traumatic inflammatory and regenerative processes along the rostro- caudal axis of the spinal cord after administration of mesenchymal stem cells: an immunohistochemical and proteomic study

Principal investigator: Dáša Čížková
Duration of project: 1.1.2015 / 31.12.2017
Project registration number: 2/0125/15

 

Annotation:

Spinal cord injury (PM) leads to sensory-motor disfunction with lifetime consequences of disability. Due to the complex pathological processes, most treatments are ineffective. The main objective is to modulate several post - traumatic factors in the segments above and below the spinal cord injury by means of cellular therapy (mesenchymal stem cells - MSCs).

We will explain the mechanisms of action of the MSC and their products in order to : i ) reduce pro-inflammatory cytokines release from microglia / macrophages in the PM and ii) stimulate nerve fibers overgrowth within in vitro and in vivo systems.

By means of mass spectrometry, immunohistochemistry and image analysis techniques, we will obtain a global map of chemokines, cytokines and trophic factors before and after application of cellular therapy. Our research in preclinical in vitro and in vivo models will contribute to the creation of new therapeutic approaches in regenerative medicine with the potential for transfer to clinics.