NBU SAV

Analysis of post-traumatic inflammatory and regenerative processes along the rostro- caudal axis of the spinal cord after administration of mesenchymal stem cells: an immunohistochemical and proteomic study

Annotation:

Spinal cord injury (PM) leads to sensory-motor disfunction with lifetime consequences of disability. Due to the complex pathological processes, most treatments are ineffective. The main objective is to modulate several post - traumatic factors in the segments above and below the spinal cord injury by means of cellular therapy (mesenchymal stem cells - MSCs).

We will explain the mechanisms of action of the MSC and their products in order to : i ) reduce pro-inflammatory cytokines release from microglia / macrophages in the PM and ii) stimulate nerve fibers overgrowth within in vitro and in vivo systems.

By means of mass spectrometry, immunohistochemistry and image analysis techniques, we will obtain a global map of chemokines, cytokines and trophic factors before and after application of cellular therapy. Our research in preclinical in vitro and in vivo models will contribute to the creation of new therapeutic approaches in regenerative medicine with the potential for transfer to clinics. 

Blood as a medium providing the tolerance in a brain after global and focal ischemic attack

Annotation:

Presented project is focused on the study of mechanisms inducting ischemic tolerance in the models of global and focal brain ischemia in rat. The main goal is determination of mechanism providing information transfer that participates on the creation of brain tolerance to ischemic condition.

Based on the knowledge that ischemic tolerance can be induced in any tissue or organ using the broad spectrum of indirect interventions, we will focus on the study of circulating blood and its compartments as the only one medium that fits to the access flexibility.

In addition to the source identification and identity of factor inducing ischemic tolerance, our intention is to describe how is this information transmitted to the nerve tissue (overcoming natural barriers), and how the machinery itself triggers induction of the tolerance in different neuronal populations affected by ischemia. For fulfillment the objectives we want to use complex strategy involving several biochemical, proteomic and histological approach.