NBU SAV

Ischemic injured rat brain tissue response to application of postconditioning: the study of ischemic tolerance acquisition with MALDI bottom up proteomic approach

Annotation:

Study of formation and possibilities of ischemic tolerance protective mechanisms participating in the brain cells defense after ischemic injury is basic idea of the project. Project is built on the finding that an end-effector for ischemic tolerance acquisition depends on second stress incidence.

It means that sublethal stress if applicated within 2 days after the lethal one can have protective effect against delayed neuronal death. Specifically, we will focus on protein profiling in the most ischemic-reperfusion injury sensitive brain areas (e.g. CA1), as well as in the relative resistant regions (dentate gyrus) by the mass spectrometry proteomic analysis. We suppose that proteomic pattern allows us to understand mechanisms, which are involved in targeted protection and survival of neurons.

Characterization of changes in protein profiles could contribute to identification of molecular processes which are participating in stress response regulation and are involved in ischemic reperfusion pathophysiology.