Axonal regeneration in biosynthetic nerve guide conduits
Annotation: In adult mammals, nerve fibers are capable of regeneration. In peripheral nerve after injury, axons from the proximal stump regrow across the site of injury into the distal stump and create new connections with their target organs. Current surgical techniques allow for a direct reconstruction of the severed nerve (end-to-end) only if the missing segment is not too large. After devastating injuries, autologous nerve grafts can be used. Synthetic nerve guides for nerve regeneration can be used as a substitute for autologous grafts. Regrowth of regenerating fibers across the synthetic nerve guides is limited to relatively short distances. This restriction is surprising, because in the distal stumps, regenerating axons are able to grow over long distances. Advances in the synthesis of biocompatible polymers provide new biomaterials with optimized properties. In the present project, we plan to use biosynthetic guides made from new elastomeric materials. The properties of the nerve guide will be modified and tested in vitro, with the goal to stimulate regeneration over long distances. The effectiveness of these modifications on regeneration process will be tested in vivo. Endogenous stimulation and exogenous application of neurotrophic factors to reduce secondary damage in model of spinal cord compression
Annotation: One of the main objectives of spinal cord injury research is to restore neural connections. The goal of present project is to examine changes in the intensity and extent of axonal damage in motor pathways and in functional recovery after spinal cord compression after 1) exogenous application of neurotrophic factors, 2 ) stimulation of endogenous neurotrophic factors by application of non-peptide agonist angiotensin AT2R, 3) application of 3D scaffold created using biodegradable composite porous membrane prepared from chitosan, and 4) combination therapy (the most effective exogenous/endogenous stimulation of growth factors and biodegradable composite). To quantify these changes, the markers for overgrowth of axons, demylinization, inflammatory response,apoptosis and oxidative/nitrosative stress will be analysed. To assess the effectiveness of the proposed therapy for signal transmission over long conductive spinal systems, the suitable markers will be applied.
Nová kombinovaná terapia na báze alginátových biomateriálov a trofických faktorov pre obnovu poranenej miechy.
Projects of the Slovak Research and Development Assistance Agency (APVV) Axonal regeneration in biosynthetic nerve guide conduits Principal investigator: Ivo Vanický Projects of the Scientific Grant Agency (VEGA) Blood as a medium providing the tolerance in a brain after global and focal ischemic attack Principal investigator: Petra Bonová __________________________________________________________________________ Analysis of post-traumatic inflammatory and regenerative processes along the rostro- caudal axis of the spinal cord after administration of mesenchymal stem cells: an immunohistochemical and proteomic study Principal investigator: Dáša Čížková __________________________________________________________________________ Endogenous stimulation and exogenous application of neurotrophic factors to reduce secondary damage in model of spinal cord compression Principal investigator: Nadežda Lukáčová __________________________________________________________________________ Impact of angiogenesis inhibition on postnatal neurogenesis Principal investigator: Marcela Martončíková __________________________________________________________________________ Ischemic injured rat brain tissue response to application of postconditioning: the study of ischemic tolerance acquisition with MALDI bottom up proteomic approach Principal investigator: Miroslava Némethová __________________________________________________________________________ Neuroprotective mechanisms of the AT2 receptor stimulation after traumatic spinal cord injury Principal investigator: Jaroslav Pavel __________________________________________________________________________ Investigation of postnatal neurogenesis in relation to neurodegeneration Principal investigator: Enikö Račeková __________________________________________________________________________ Influence of electromagnetic radiation on some organs of sexually immature rats Principal investigator in SAS: Enikö Račeková
International projects Study of the neuroprotective potential of P2X7 and CALHM1 channels in ischemia Principal investigator: Miroslav Gottlieb __________________________________________________________________________ Nová kombinovaná terapia na báze alginátových biomateriálov a trofických faktorov pre obnovu poranenej miech Principal investigator in SAS: MVDr. Dáša Čížková, DrSc.
Projects of EU Structural Funds MEDIPARK - University biomedical scientific park of P.J. Safarik University Principal investigator: Nadežda Lukáčová Influence of electromagnetic radiation on some organs of sexually immature rats
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Institute of Neurobiology
Slovak Academy Of Science
Slovak Republic