NBU SAV

The therapeutic effects of stem cells conditioned medium on the repair of the spinal cord damaged tissue: a comparative ex vivo study

Annotation: Spinal cord injury is a neurodegenerative disease of the CNS, which leads to damage and death of the spinal cord cell population, namely neurons, oligodendrocytes, astrocytes. Cell therapy is a key area of regenerative medicine to find suitable sources of transplanted cells or their products, allowing restoration of damaged tissue. The aim of the present project is to establish an ex vivo model of traumatic injured CNS tissue in the form of organotypic spinal cord slices. Using this model we will investigate the effects of the conditioned medium of the stem cells (mesenchymal stem cells from bone marrow and adipose tissue, neural progenitors) on the repair of damaged rat spinal cord tissue. We compare the effectiveness of different conditioned media on the base of survival and proliferative potential of glial cell population, neurons and nerve fibers overgrowth. We suppose, that the ex vivo model of mechanical damage will be a good method for the study of potential therapeutic strategies in CNS damage.